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Detect & identify biomolecules -                                                                                          
        Advance the education & training of 'omics' sciences for high schools
From the laboratory of Dr. Sixue Chen at the University of Florida        

Introduction to Proteomics

I. Protein Introduction

II. Protein Structure

III. Protein Snthesis

IV. Proteomes

V. Isolating Proteins

VI. Identifying Proteins

VII. PMF Technology

How does a mass spectrometer determine the mass of a molecule?
  In a laser-desorption mass spectrometer, pulses of light from a laser ionize proteins (or peptides) that are absorbed onto a metal target. An electric field accelerates the molecules toward the detector. The time it takes for each molecule to reach the detector is inversely proportional to the mass-to-charge ratio (m/z) of the molecule. In this type of ionization, each molecule usually only takes one charge, therefore the time to the detector is inversely proportional to the mass of the molecule.