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Detect & identify biomolecules -                                                                                        
Advance the education & training of 'omics' sciences for high schools
      
From the laboratory of Dr. Sixue Chen at the University of Florida        

   

2012 ICORE Teachers "Omics" Workshop

(July 13 -14, 2012 in ICBR Education and Training Lab, Chen Lab and Polfer Lab)

Sponsored by the National Science Foundation and the UF CPET program

 

 

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ICORE 2011 Participants pictured in the front of the Emerging Pathogens Institute

 

 

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Introduction of the Proteomics Lab by Ms. Drew Joseph from ICORE and Dr. Sixue Chen, Associate Professor from Biology Department

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Dr. Chen introduces the plant materials and the objectives of the proteomics experiments

 

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Sample preparation

 

 

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Protein extraction from control tomato leaves and pathogen-infected tomato leaves

 
 

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Dr. Chen chats with the teachers about sample preparation procedures

 

 
 

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Tong Zhang, a graduate student from Chen lab works with a teacher

 

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Lan Yang and Da Wang, two graduate students from Polfer lab work with the teachers

 

 
 

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An interactive lab

 
 

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Michelle Chin, a high school student attends the workshop, Tong Zhang is helping with sample preparation

 

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Jennifer Parker, a graduate student from Chen lab talks with the teachers

 

 
 

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Drew and an undergraduate student work with the teachers

 

 
 

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Tong Zhang talks with a teacher

 

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Huizhuan Yan, a graduate student from Chen lab works with the teachers

 

 
 

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A lot of hands-on experiments

 
 

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Dr. Chen gives a "omics" and systems biology lecture

 
 

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Jennifer and Huizhuan demonstrate protein gel electrophoresis

 
 

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Dr. Chen and Jennifer with the teachers

 
 

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Loading the samples onto the gel

 

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Gel electrophoresis is COOL

 

 
 

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A little discussion about stuff

 
 

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Drew amd Huizhuan help with the gel electrophoresis lab

 
 

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Jennifer and Tong help with the gel electrophoresis lab

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How to take the gel out?

 
 

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Take a good picture of the gel

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A nice separation of proteins, which band to cut?

 
 

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Stain-free gels work really well

 

 
 

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Mass spectrometry lecture and demonstration by Drs. Cecilia Silva-Sanchez and Chen

 

 
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Protein in-gel digest spotting on MALDI sample plate

 
 

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Acquiring and inspecting MALDI-TOF peptide fingerprints

 

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MALDI-TOF/TOF instrument used for the workshop

 

 
 

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Acquiring and analyzing more peptide mass fingerprints for protein identification

 
 

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Dr. Chen introduces nanoflow LC-MS/MS

 

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Quadrupole ion trap and nanoflow HPLC technology

 

 
 

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LC-MS/MS data inspection

 
 

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State-of-the-art Orbitrap technology

 
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A lot of interactions

 
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Proteomics workshop is FUN

 
 

For more pictures, please click here: http://www.cpet.ufl.edu/ICORE/ICORE2012/PhotoGallery.html

 
 
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courtesy of Dr. Sixue Chen's laboratory at the University of Florida, Gainesville, Florida


This site was last modified: June 17, 2012 1:39 PM.